Food-borne pathogens survival of Yersinia enterocolitica and molecular analysis of 16SrRNA sequencing of Salmonella typhi & Salmonella enterica from frozen buffalo meat in India

French Bacteriologist Alexander Yersinia in 1894 first described eniterocolitica as causative organisms for food-borne illness. enterocolitica is an enteropathogenic organism and causes “yersiniosis” that denotes infections such as, gastroenteritis infants young children it considered bacillary dysentery or campylobacter - like enterocolitis with abdominal pain diarrhea. Even give rise to acute terminal ileitis mesenteric lymphadenitis, cause septicemia high fatality rate, abortion, pneumonia, ileocaecal appendix, proximal colon level, enterocolitis, immunological complications erythema nodosum, polyarthritis, Reiter’s syndrome, some time leading death etc. In frozen buffalo meat at very freezing temperature the common isolated can spoilage. Y. gram negative, pleomorphic, asporogenous short, capsulated, motile peritrichous flagella microscopically grown 22oC. aerobe facultative anaerobe actively porn zoonosis. Salmonella species also a risky food borne pathogen typhoid salmonellosis. present research investigation 51 samples of processed microbiologically whereas 39 are positive 11 negative enterocolitica. Hence, It observed load tolerance littile higher meat. MacConkey agar showed small, irregular, pinpointed, pinkish reddish pale pink reject red color lactose colonies 37oC form pinpoint Antibiotic assay test revealed 10µg amoxyclave disc sensitive but, 5µg methicillin resistant The from specific media (e.g. XLD, BGA, BSA, HEA) subjected molecular analysis 16SrRNA sequencing identified typhi strain T9 16S ribosomal RNA gene, partial sequence enterica subsp. serovar enteritidis DY60 sequence, spoilage low threaten public health safety. overall result indicates poor management hygiene abattoir inefficient cleaning condition augment serious issue reassessment importance.